The National Quantum Initiative (NQI) Act became Public Law in December 2018 to accelerate American leadership in quantum information science and technology. This is the third Annual Report on the NQI Program, as required by the NQI Act. The United States has invested in fundamental Quantum Information Science (QIS) R&D, with core efforts underway in over a dozen agencies. This report recognizes annual highlights of the entire Federal QIS R&D ecosystem. In addition, brief summaries of agency efforts and progress on cross-cutting QIS policy topics are included within the report.